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. 2021 Jul 14;11:14500. doi: 10.1038/s41598-021-93642-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histological evaluation of wound healing. (a) Pathological images of the wound on day 14 (HE stain, scale bar = 500 μm). (b) Thickness of formed granulation tissue in each treated group on day 14. The granulation tissue in the animal group of ASC spheroids NS for RU is about 4.8-times thicker than that in the Untreated RU group (granulation thickness: 1.41 ± 0.27 vs. 0.29 ± 0.18 mm, p < 0.01). (c) Length of regenerative epithelium in each treated group. The regenerative epithelium in the animal group of the ASC spheroids NS for RU is about 2-times longer than that in the Untreated RU group (regenerative epithelial length: 2.92 ± 1.21 mm vs. 1.12 ± 0.30 mm, p < 0.01).