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. 2021 Jan 15;46(9):1554–1564. doi: 10.1038/s41386-020-00919-x

Fig. 2. Intra-BLA AM251 administration after memory reconsolidation does not alter drug context-induced cocaine seeking 3 days later.

Fig. 2

A Experimental timeline. After cocaine self-administration training in one context (cocaine-paired context, Context A) and extinction training in a different context (extinction context, Context B), rats received bilateral intra-BLA administration of the CB1R antagonist, AM251 (0.3 µg/0.5 µL per hemisphere; n = 8) or vehicle (n = 6) six hours after the 15-min cocaine-memory retrieval session (after memory reconsolidation was completed). After two additional extinction sessions in the extinction context with ≤25 active lever responses, cocaine-seeking behavior was tested in the cocaine-paired context. B Schematic of cannula placements. Symbols represent the most ventral point of injection cannula tracts for rats that received vehicle or AM251. C Active-lever responses (mean ± SEM) during cocaine self-administration (last 10 days) and extinction training prior to AM251 or vehicle treatment. D Active-lever responses (mean ± SEM) during the memory-retrieval session (before treatment) and upon first re-exposure to the extinction context and the cocaine-paired context (after treatment) at test. E Time course of active-lever responses (mean ± SEM) at test in the cocaine-paired context. Symbols: ANOVA, #context main effect, p < 0.05 ‡time simple-main effects, Tukey’s tests, intervals 1  > intervals 2–6, ps < 0.05.