Fig. 1.

Culture and identification of MSCs and DMSCs. a The morphology of MSCs. Scale bar represents 100 μm. b MSCs were identified as CD45⁻CD11b⁻CD31⁻CD86⁻CD29⁺CD44⁺Sca-1⁺. c Morphology of living MSCs and DMSCs. Scale bar represents 100 μm. d Identification of living and dead MSCs by Trypan blue. Scale bar represents 100 μm. e Living and dead MSCs were stained using LIVE/DEAD^TM Near-IR Dead Cell Stain Kit and were analyzed by flow cytometry. f The apoptosis of MSCs and DMSCs detected by flow cytometry. g The PI staining of MSCs and DMSCs. Scale bar represents 50 μm. h, i The expression of cleaved caspase 3 (h) and Cathepsin B (i) in MSCs and DMSCs. Scale bar represents 10 μm. C-Cas 3, cleaved caspase 3