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. 2021 Jul 1;9:686362. doi: 10.3389/fbioe.2021.686362

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Copyright © 2021 Bessonnet, Mariage, Petit, Pellouin, Debard, Zaparucha, Vergne-Vaxelaire and de Berardinis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Conversion of 3-phenylpropionitrile into 3-phenylpropionic acid by Nit_phym purified by heat treatment. Reaction conditions: 10 mM 3-phenylpropionitrile, 100 mM potassium phosphate buffer pH 7.5, 1 mM DTT, 0.07 mg⋅mL^{– 1} Nit_phym, 600 rpm, 30°C. Reactions were analyzed by UHPLC-UV with condition B. Red error bars represent the standard deviation of two independent experiments.