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. 2021 Jul 1;9:686362. doi: 10.3389/fbioe.2021.686362

FIGURE 8.

FIGURE 8

Effect of 3-phenylpropionitrile concentration on conversion. Reaction conditions: 100–600 mM 3-phenylpropionitrile, 100 mM potassium phosphate buffer pH 7.5, 1 mM DTT, 20% (vol/vol) DMSO, 0.1 mg⋅mL– 1 Nitphym, 400 rpm, 50°C, 48 h. The amount of 3-phenylpropanoic acid formed was determined by UHPLC-UV monitoring (condition B) and deduced from calibrations curves.