FIGURE 8.
Effect of 3-phenylpropionitrile concentration on conversion. Reaction conditions: 100–600 mM 3-phenylpropionitrile, 100 mM potassium phosphate buffer pH 7.5, 1 mM DTT, 20% (vol/vol) DMSO, 0.1 mg⋅mL– 1 Nitphym, 400 rpm, 50°C, 48 h. The amount of 3-phenylpropanoic acid formed was determined by UHPLC-UV monitoring (condition B) and deduced from calibrations curves.
