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. 2021 Jul 15;192:113503. doi: 10.1016/j.bios.2021.113503

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© 2021 Published by Elsevier B.V.

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Fig. 2 — Optimization of the flap probe-based isothermal nucleic acid amplification. (A) Feasibility verification of fluorescence; (B) agarose electrophoresis results (corresponding to the curves in A) for rotavirus (line 1 and 3) and Chlamydia trachomatis (line 2 and 4) amplicons; (C) photographs of the amplified products under white light (left) and ultraviolet light (right); (D) fluorescence by flap sequence type; (E) fluorescence by flap sequence length. Notes: A, adenine; C, cytosine; G, guanine; T, thymine; Ct, Chlamydia trachomatis; RV, rotavirus.