Table 2.
Single-cell studies of human and mouse atherosclerotic plaques
| Sample and model | Cell type focus | Analysis (platform) | Ref. |
|---|---|---|---|
| Studies in mice | |||
| Atherosclerotic aorta from Apoe−/− or Ldlr−/− mice | Immune cells | CyTOF; scRNA-seq (10× Genomics) | 99 |
| Atherosclerotic aorta from Cx3cr1CreERT2-EYFP/+Rosa26tdTomato/+ mice injected with AAV-mPCSK9 | Macrophages | scRNA-seq (10× Genomics) | 131 |
| Normal aorta and atherosclerotic aorta from Apoe−/− or Ldlr−/− mice | CD45+ immune cells, macrophages | scRNA-seq (10× Genomics) | 98 |
| Normal aorta and atherosclerotic aorta from Apoe−/− mice | Vascular smooth muscle cells | scRNA-seq (Fluidigm C1, Smart-Seq2, 10× Genomics) | 155 |
| Atherosclerotic aortas from Apoe−/− or Ldlr−/− mice | CD45+ immune cells, macrophages | FACS combined with scRNA-seq (10× Genomics) | 130 |
| Aortas from Apoe−/− mice fed a chow or a high-fat diet | CD45+ immune cells, macrophages | CyTOF | 97 |
| Aortas from Apoe−/− mice treated with pro-efferocytosis nanoparticles | CD45+ immune cells, macrophages | scRNA-seq (10× Genomics) | 156 |
| Aortas from Apoe−/− mice fed a chow or a Western diet | CD4+ T cells | scRNA-seq (10× Genomics) | 157 |
| Studies in humans | |||
| Carotid atherosclerotic plaque and peripheral blood mononuclear cells | CD45+ immune cells, macrophages, T cells | CD45+ bead-based enrichment combined with CyTOF, CITE-seq and scRNA-seq (10× Genomics) | 25 |
| Carotid atherosclerotic plaque | Immune cells, vascular smooth muscle cells, endothelial cells | FACS combined with scRNA-seq (CEL-seq2) and scATAC-seq | 101 |
| Studies in humans and mice | |||
| Human atherosclerotic coronary arteries and mouse atherosclerotic aorta | Immune cells, vascular smooth muscle cells | FACS combined with CITE-seq and scRNA-seq (10× Genomics) | 142 |
AAV, adeno-associated virus; CITE-seq, cellular indexing of transcriptomes and epitopes by sequencing; CyTOF, cytometry by time of flight; FACS, fluorescence-activated cell sorting; scATAC-seq, single-cell assay for transposase-accessible chromatin using sequencing; scRNA-seq, single-cell RNA sequencing.