Table 1.

Primers and probes used in this study (Corman et al. 2020; Waggoner et al. 2020)

Organisms	Target gene	Sequence (5′–3′)	Cycling parameters
SARS-CoV-2	Probe and primer ORF1a/b	FACAGGTGGAACCTCATCAGGAGATGC-BBQ F-GTGARATGGTCATGTGTGGCGG R-CARATGTTAAASACACTATTAGCATA	55°C 10′ 94°C 3′ 94°C 15″ 58°C 30″ 45x.
	Primer and probe N gene	F-AAATTTTGGGGACCAGGAAC R-TGGCAGCTGTGTAGGTCAA PFAM-ATGTCGCGCATTGGCATGGA-BHQ	55°C 10′ 94°C 3′ 94°C 15″ 58°C 30″ 45x