Table 3. DSF Thermal Shifts for CkGE15A in the Presence of Various Oligosaccharidesa.
| oligosaccharide | ligand concentration (mM) | Ti1 (°C) ± SD | Ti2 (°C) ± SD |
|---|---|---|---|
| none | – | 72.1 ± 0.21 | 81.3 ± 0.37 |
| maltose | 10 | 72.3 ± 0.23 | 81.6 ± 0.24 |
| cellobiose | 10 | 72.4 ± 0.10 | 81.7 ± 0.32 |
| xylobiose | 10 | 72.4 ± 0.10 | 81.4 ± 0.22 |
| xylotriose | 10 | 72.1 ± 0.17 | 81.3 ± 0.22 |
| xylotetraose | 10 | 72.0 ± 0.07 | 81.1 ± 0.33 |
| aldopentauronic acid | 10 | 72.5 ± 0.23 | 79.2 ± 0.49 |
| benzylglucuronate | 10 | 72.8 ± 0.32 | 79.3 ± 0.49 |
| glucuronic acid | 10 | 72.2 ± 0.28 | 81.5 ± 0.75 |
a
Ti values significantly different (three standard deviations) from that of the control without a ligand are shown in bold.