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. 2021 Jul 1;12:693007. doi: 10.3389/fphys.2021.693007

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Copyright © 2021 Kobayashi, Eguchi, Tamai, Fukuda, Tempaku, Izuoka, Iwasa, Takei and Togashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification and detection of EV source from circulating EV at T₀. (A) Purification of circulating EVs using qEV column. (B) Representative immunoblotting analysis of CD9, CD81, ALIX, TSG101, syntenin-1, annexin V, α-skeletal muscle actin (α-skeletal MA), asialoglycoprotein receptor 1 (ASGPR1), perilipin, and apolipoprotein A1 (ApoA1) in isolated EVs from six subjects. (C) EV size of isolated EVs from six subjects by nano tracking analysis (NTA). Bar graph of isolated EV size from NTA analysis.