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. 2021 Jul 1;12:693007. doi: 10.3389/fphys.2021.693007

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Copyright © 2021 Kobayashi, Eguchi, Tamai, Fukuda, Tempaku, Izuoka, Iwasa, Takei and Togashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detected EV proteins. (A) Changes in circulating EV number at pre-HIIT-Ex, T₀, and T₃₀ in three subjects using proteomic analysis. (B) Detected protein number at pre-HIIT-EX, T₀, and T₃₀. (C) Heatmap of identified proteins with significant changes in isolated circulating EVs at pre-HIIT-Ex, T₀, and T₃₀. (D) Change in EV protein values at pre-HIIT-Ex, T₀, and T₃₀. Fibrinogen gamma chain (FGG), catalase (CAT), carbonic anhydrase (CA-1), and peroxiredoxin II (PRDX-II). Kruskal-Wallis test was used for evaluating statistical significance among the three sampling points. ^****p < 0.0001, ^∗∗p < 0.01, ^∗p < 0.05. Pre: pre-HIIT-Ex.