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. 2021 Jul 1;9:684505. doi: 10.3389/fcell.2021.684505

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Copyright © 2021 Ji, Tian, Gao, Zang, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-8 activates NFs in multiple signal pathways. (A) Measurement of IL-8 in CAF CM and NF CM by ELISA. (B,C) Detection of CXCR1 and CXCR2 in NFs and CAFs by Western blotting and qRT-PCR. Triple independent experiments were carried out, and representative results are shown. (D) Analysis of AKT/ERK-associated proteins including CXCR1/2, AKT, pAKT, ERK, pERK, and angiogenesis-related proteins (VEGF and TSP-1) by Western blotting in NFs treated with CAF CM, CAF CM + anti-IL-8, and IL-8 alone. β-Actin was used as a loading control. *p < 0.05, ***p < 0.001. IL, interleukin; NFs, normal ovarian fibroblasts; CAFs, cancer-associated fibroblasts; CM, conditioned media; VEGF, vascular endothelial growth factor.