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IL-8 secreted from CAFs promotes cancer cell stemness. (A) Spheroids formed by HEY-A8 cells treated with IgG, CAF CM, CAF CM+anti-IL-8, or IL-8. (B) Analysis of KLF4, SOX2, and CD133 expression by Western blotting in HEY-A8 cells treated with IgG, CAF CM, CAF CM+anti-IL-8, or IL-8. GAPDH was used as a loading control. IL, interleukin; CAFs, cancer-associated fibroblasts. *p < 0.05, **p < 0.01, ***p < 0.001.
