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. 2021 Jul 1;9:684505. doi: 10.3389/fcell.2021.684505

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Copyright © 2021 Ji, Tian, Gao, Zang, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-8 enhances ovarian cancer cell stemness and chemoresistance. (A) Examination of IL-8 expression in IL-8 overexpression and silencing ovarian cancer cells by qRT-PCR. (B) Measurement of IL-8 secreted from overexpression and silencing cells by ELISA. (C) Spheroids formed by IL-8 overexpression and silencing ovarian cancer cells in 3-D culture. (D) Survival rates of ovarian cancer cells expressing IL-8 shRNA or cDNA tested by CCK-8 following the treatment with cisplatin. OD values were measured after treatment with cisplatin at 48 h. *p < 0.05, **p < 0.01, ***p < 0.001. IL, interleukin; CCK-8, Cell Counting Kit-8; OD, optical density.