Figure 3.

Cellular location of native LIC13086 in L. interrogans serovar Copenhageni. (A) L. interrogans intact cells or soluble lysates were immobilized onto ELISA plates. Primary antiserum against LipL31, DnaK, LipL46, or LIC13086 were added and incubated for 1 h at 37°C. The detection was performed with HRP-conjugated goat anti-mouse IgG (1:5,000). Data represent the means ± the standard deviation of triplicates, representative of three independent experiments. (B) L. interrogans were fixed with 2% paraformaldehyde at 30°C for 1 h and primary antiserum against LipL31, LipL46, or LIC13086 was added for 1 h at 30°C. After incubation, FITC-conjugated goat anti-mouse IgG (1:50) was added for 16 h at 4°C. Fluorescence measurements were performed in a BD FACSCanto II. *Indicates significant difference calculated by Student t-test with p-values < 0.05.