Figure 7.

Characterization of rLIC13086 interaction with fibrinogen. (A) Coated fibrinogen (1 μg) was incubated with different concentrations of rLIC13086 (0–5 μM) for 2 h at 37°C and detected with HRP-conjugated anti-HisTag mAb (1:10,000). (B) rLIC13086 was incubated with human fibrinogen for 2 h at 37°C, thrombin was added, and fibrin clot formation was recorded for 30 min by measuring absorbance at 595 nm. C+: Positive control with only fibrinogen and thrombin; C−: Negative control without fibrinogen. (C) rLIC13086 was immobilized and incubated with increasing concentrations of NHS. The interaction was assessed by the addition of goat anti-human fibrinogen (1:10,000) followed by HRP-conjugated anti-goat IgG (1:50,000). BSA was used as negative control. The bars represent the means of three different experiments in triplicates with the corresponding standard deviation. * Indicates significant difference calculated by Student t-test with p-values < 0.05.