Figure 9.

Characterization of rLIC13086 interaction with complement system components. (A) rLIC13086 immobilized was co-incubated with C7, C8, or C9 (1 μg) and different concentrations of heparin (0–500 μg/ml) for 2 h at 37°C. Detection was performed with HRP-conjugated anti-HisTag mAb (1:10,000). (B) rLIC13086 was immobilized and incubated with increasing concentrations of NHS. The interaction was assessed by the addition of each goat anti-human complement component (1:10,000) followed by HRP-conjugated anti-goat IgG (1:50,000). BSA was used as negative control. The bars represent the means of three different experiments in triplicates with the corresponding standard deviation. * Indicates significant difference calculated by Student t-test with p-values < 0.05.