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. 2021 Jun 15;40(14):e106355. doi: 10.15252/embj.2020106355

Table 2.

Antibodies used in this study.

Antibodies Source Reference Application (Dilution)
anti‐PrimPol, polyclonal Generated in house Mourón et al, (2013)

WB (1:1,000)

IP (neat serum)

anti‐PrimPol, monoclonal Generated in house this work

WB (neat tissue culture supernatant, TCSN)

IF (neat TCSN)

anti‐CTFC Millipore 07‐729 WB (1:1,000)
anti‐TUBULIN Sigma T6199 WB (1:1,000)
anti‐H3 Abcam 1791 WB (1:10,000)
anti‐MEK2 BD Biosciences 610236 WB (1:1,000)
anti‐FANCD2 Fanconi Anemia Research Fund (FARF) B9699 IF (1:100)
anti‐FANCD2 Novus Biologicals NB100‐182

WB (1:10,000)

IF (1:300)

anti‐BLM Bethyl Laboratories A300‐110A WB (1:1,000)
anti‐BLM GeneTex GTX25446 WB (1:100)
anti‐RMI1 Abcam Ab70525 WB (1:1,000)
anti‐RMI1 Novus Biologicals NB100‐1720 WB (1:1,000)
anti‐RMI2 Novus Biologicals NBP1‐89962 WB (1:1,000)
anti‐TOP3A Proteintech 14525‐1‐AP WB (1:1,000)
anti‐RPA2 Cell Signaling 2208S WB (1:1,000)
anti‐phospho‐RPA2 ser4/8 Cell Signaling 54762S WB (1:1,000)
anti‐MCM3 Méndez Laboratory Ibarra et al, (2008) WB (1:1,000)
anti‐V5 Invitrogen 46‐0705

WB (1:1,000)

IF (1:500)

anti‐SMC1 Losada’s laboratory Kojic et al, (2018) WB (1 μg/ml)
anti‐FANCA Bethyl Laboratories A301‐980A WB (1:2,000)
anti‐FANCM Bethyl Laboratories A302‐637A WB (1:4,000)
anti‐FANCM Sigma SAB1407805 IF (1:100)
anti‐Neil3 Proteintech 11621‐1‐AP WB (1:1,000)
anti‐γH2AX Merk Millipore 05‐636 IF (1:300)
anti‐Ki67 Cell Signaling 12202 IHC
anti‐CldU (rat monoclonal anti‐ BrdU) Abcam ab6326 IF (1:100)
anti‐IdU (mouse monoclonal anti‐BrdU) BD biosciences 347580 IF (1:100)
anti‐ssDNA Millipore MAB3034 IF (1:300)
anti‐Dig Abcam ab76907 IF (1:1,000)
anti‐rabbit IgG AF‐488 (chicken) Invitrogen Molecular Probes A‐21441 IF (1:300)
anti‐rat IgG AF‐594 (goat) Invitrogen Molecular Probes A‐11007 IF (1:300)
anti‐mouse IgG2a AF‐647 (goat) Invitrogen Molecular Probes A‐21241 IF (1:300)
anti‐goat IgG AF‐647 (donkey) Invitrogen Molecular Probes A‐21447 IF (1:1,500)