Table 2.
Antibodies | Source | Reference | Application (Dilution) |
---|---|---|---|
anti‐PrimPol, polyclonal | Generated in house | Mourón et al, (2013) |
WB (1:1,000) IP (neat serum) |
anti‐PrimPol, monoclonal | Generated in house | this work |
WB (neat tissue culture supernatant, TCSN) IF (neat TCSN) |
anti‐CTFC | Millipore | 07‐729 | WB (1:1,000) |
anti‐TUBULIN | Sigma | T6199 | WB (1:1,000) |
anti‐H3 | Abcam | 1791 | WB (1:10,000) |
anti‐MEK2 | BD Biosciences | 610236 | WB (1:1,000) |
anti‐FANCD2 | Fanconi Anemia Research Fund (FARF) | B9699 | IF (1:100) |
anti‐FANCD2 | Novus Biologicals | NB100‐182 |
WB (1:10,000) IF (1:300) |
anti‐BLM | Bethyl Laboratories | A300‐110A | WB (1:1,000) |
anti‐BLM | GeneTex | GTX25446 | WB (1:100) |
anti‐RMI1 | Abcam | Ab70525 | WB (1:1,000) |
anti‐RMI1 | Novus Biologicals | NB100‐1720 | WB (1:1,000) |
anti‐RMI2 | Novus Biologicals | NBP1‐89962 | WB (1:1,000) |
anti‐TOP3A | Proteintech | 14525‐1‐AP | WB (1:1,000) |
anti‐RPA2 | Cell Signaling | 2208S | WB (1:1,000) |
anti‐phospho‐RPA2 ser4/8 | Cell Signaling | 54762S | WB (1:1,000) |
anti‐MCM3 | Méndez Laboratory | Ibarra et al, (2008) | WB (1:1,000) |
anti‐V5 | Invitrogen | 46‐0705 |
WB (1:1,000) IF (1:500) |
anti‐SMC1 | Losada’s laboratory | Kojic et al, (2018) | WB (1 μg/ml) |
anti‐FANCA | Bethyl Laboratories | A301‐980A | WB (1:2,000) |
anti‐FANCM | Bethyl Laboratories | A302‐637A | WB (1:4,000) |
anti‐FANCM | Sigma | SAB1407805 | IF (1:100) |
anti‐Neil3 | Proteintech | 11621‐1‐AP | WB (1:1,000) |
anti‐γH2AX | Merk Millipore | 05‐636 | IF (1:300) |
anti‐Ki67 | Cell Signaling | 12202 | IHC |
anti‐CldU (rat monoclonal anti‐ BrdU) | Abcam | ab6326 | IF (1:100) |
anti‐IdU (mouse monoclonal anti‐BrdU) | BD biosciences | 347580 | IF (1:100) |
anti‐ssDNA | Millipore | MAB3034 | IF (1:300) |
anti‐Dig | Abcam | ab76907 | IF (1:1,000) |
anti‐rabbit IgG AF‐488 (chicken) | Invitrogen Molecular Probes | A‐21441 | IF (1:300) |
anti‐rat IgG AF‐594 (goat) | Invitrogen Molecular Probes | A‐11007 | IF (1:300) |
anti‐mouse IgG2a AF‐647 (goat) | Invitrogen Molecular Probes | A‐21241 | IF (1:300) |
anti‐goat IgG AF‐647 (donkey) | Invitrogen Molecular Probes | A‐21447 | IF (1:1,500) |