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. 2021 May 25;40(14):e107294. doi: 10.15252/embj.2020107294

Figure EV1. TMD and CTD arrangement in KCC1Δ19 and KCC3b‐PM, in comparison to DrNKCC1 and CTD‐free KCC1 (Related to Fig 1).

Figure EV1

  • A
    Cartoon representation of the KCC3b‐PM dimer with inset illustrating the TMD of one subunit. The transporter core domain is shown in orange, the scaffold domain in wheat.
  • B, C
    Slab view of the transporter domain of KCC3 (construct: PM) (B) and KCC1 (construct: Δ19) (C) in surface representation, highlighting the inward‐open conformations with intracellular accessible vestibules.
  • D, E
    Extracellular view of the TMD dimer of KCC3b‐PM (D) and DrNKCC1 (E).
  • F, G
    Extracellular view onto central TM11/TM12 helices and CTD, highlighting counter‐clockwise twisted CTD for KCC3b‐PM (F) and clockwise twisted CTD for DrNKCC1 (G).
  • H
    Extracellular view onto TMD dimer illustrating the alternative dimer interface observed in CTD‐free KCC1 (6KKR).
  • I
    Front view on CTD‐free KCC1 dimer detailing molecular interactions (inset) between IL8 (blue helix) and TM12 characteristic for the configuration in 6KKR.