Figure EV1. RT–qPCR analysis of sams mRNA isoforms in fed and unfed animals.

• A, B
  Relative amounts of sams‐3 (column 1), sams‐4 (column 2), and sams‐5 (column 3) mRNA isoforms as well as sams‐1, sams‐2, fat‐7, and acs‐2 mRNAs (column 4) compared to eef‐1A.1 (n = 3, mean ± standard error of mean) in the smg‐2 (yb979) mutant (A) and in N2 (B). The same RT products were analyzed in Fig 2B. None, S‐complete medium alone; OP50, OP50 in S‐complete (OD₆₀₀ = 10.0); +Eme, OP50 in S‐complete supplemented with 10 mg/ml emetine. Sequences of the primers are available in Appendix␣Table S3.