Figure EV3. RT–qPCR analysis of sams mRNA isoforms in smg‐2 and smg‐2; sams‐5; sams‐1 mutants fed in the presence of L‐Met or cycloleucine.

• A–C
  Relative amounts of sams‐3 (A), sams‐4 (B), and sams‐5 (C) mRNA isoforms compared to eef‐1A.1 (n = 3, mean ± standard error of mean) in smg‐2 (yb979) and smg‐2 (yb979); sams‐5 (gk147); sams‐1 (ok2946) mutants. The same RT products were analyzed in Fig 3A. None, S‐complete medium alone; OP50, OP50 in S‐complete (OD₆₀₀=10.0); +Met, OP50 in S‐complete supplemented with 25 mM L‐Met; +cLeu, OP50 in S‐complete supplemented with 25 mM cycloleucine. *P < 0.05; **P < 0.01 (Student's t‐test). Sequences of the primers are available in Appendix␣Table S3.
• D
  Relative amounts of sams‐1 mRNA and sams‐3 and sams‐4 productive mRNA isoforms compared to eef‐1A.1 (n = 3, mean ± standard error of mean) in L1 larvae of N2 and the sams‐1 (ok2946) mutant incubated without (None) or with OP50 in S‐complete (OD₆₀₀ = 10.0) for 3 h at 20°C.