Figure EV4. Expression of SAMS‐3 and SAMS‐4 is upregulated in a mett‐10 single mutant.

• A
  Relative amounts of sams‐1, sams‐3, and sams‐4 productive mRNAs compared to eef‐1A.1 (n = 3, mean ± standard error of mean) in N2 and the mett‐10 (ok2204) mutant. Synchronized L1 larvae of each strain were incubated in S‐complete medium alone (None) or with OP50 (OD₆₀₀ = 10.0) for 3 h at 20°C.
• B
  Western blot analysis of SAMS‐1, SAMS‐3, and SAMS‐4 during larval development of N2 (w) and the mett‐10 (ok2204) mutant (m). Synchronized L1 larvae of each strain were incubated with OP50 at 20°C and subjected to Western blot analysis at indicated time points. Coomassie Brilliant Blue (CBB) staining was used as a loading control. Representative images of three replicated experiments are shown. Note that upregulation of SAMS‐1 in both strains during larval development is consistent with feeding‐induced upregulation of sams‐1 mRNA shown in (A).