Figure EV2. CENATAC's congression phenotype is not the result of a multipolar mitotic spindle.

• A
  Representative stills of HeLa^{EGFP‐AID‐CENATAC} cells expressing H2B‐mNeon and depleted of GAPDH. Microtubules were visualized with SiR‐Tubulin. Scale bar, 5 μm. Time in hours. See also Movies EV1 and EV2.
• B
  Quantification of the mitotic phenotype and multipolar spindle formation in time in cells treated as in (A) (three biological replicates, > 44 cells in total).
• C
  Representative immunofluorescence images of HeLa^{EGFP‐AID‐CENATAC} cells depleted of GAPDH or CENATAC and stained with antibodies against Centrin1 and Tubulin. IAA, 3‐indoleacetic acid. Scale bar, 5 μm.
• D
  Quantification of the amount of centrioles per mitotic cell treated as in (C) (three biological replicates, > 60 cells in total).

Data information: In (B, D), data are presented as mean ± SEM. P‐values were calculated with unpaired Student's t‐tests.