Figure 3.

The TAMys facilitate tumor progression and metastasis by IL1B. (A and B) Colon26 cells were stimulated with IL1B (5 ng/mL), or the TAMys in the presence of anti-IL1B mAb or mouse IgG (1 µg/mL) for 5 days before assays. Tumor invasion and adhesion (n=3; A). Intracellular generation of BODIPY⁺ lipid droplets in tumor cells (n=3; B). (C) The TAMys promote tumor progression and metastasis. Colon26 cells (3×10⁵) were subcutaneously coinjected with CD11b⁺ cells (3×10⁵) followed by intravenous injection with Colon26 cells (2×10⁵), and 4 days later, the subcutaneous tumors were injected with anti-CTLA4 mAb and/or anti-IL1B mAb (100 µg). On day 15 after tumor implantation, tumor tissues and lungs were harvested from the mice for histological observation of tumor lipidization and metastatic tumor nodules (n=5). *P<0.01, **p<0.05 versus control group. Graphs show means±SDs. Scale=50 µm. Representative data of four independent experiments. TAMys, tumor/aspirin-associated myeloid cells.