Table 6.

Relative gene expression differences between light and average BW newborn littermates of sows fed diets with total ITM and Replace¹

Experimental diets2	Function	Response	Gene	Light	Average	Contrast statistic3	P-value	FDR
ITM	Barrier function	Trefoil factor 3	TFF3	0.93	1.33	1.712	0.207	0.650
	Immune response	Fatty acid hydrolase domain containing 2	FAXDC2	1.70	3.12	8.356	0.010	0.138
		Guanylate binding protein 1	GBP1	0.32	0.06	3.944	0.062	0.319
		Heat shock protein 1	HSPB1	1.27	1.81	1.338	0.002	0.083
		Peroxisome proliferative activated receptor gamma, coactivator 1 alpha	PPARGC1α	2.87	4.79	7.799	0.012	0.138
		Interleukin 6	IL6	1.18	1.44	0.437	0.517	0.820
		Tumor necrosis factor alpha	TNFα	2.67	3.64	0.855	0.367	0.650
	Digestive enzyme	Aminopeptidase-N	ANPEP	0.61	0.72	4.528	0.047	0.311
		Indoleamine 2,3-dioxygenase	IDO1	1.40	4.72	4.973	0.040	0.310
		Sucrase-isomaltase	SI	7.27	55.70	9.834	0.006	0.131
	Nutrient transporter	Solute carrier family 39 (Zn transporter) member 4	SLC39A4	1.31	2.20	5.704	0.028	0.258
	Stress enzyme	Hydroxysteroid (11-beta) dehydrogenase 1	HSD11B1	2.88	1.92	3.970	0.062	0.319
Replace	Barrier function	Trefoil factor 3	TFF3	1.80	1.06	6.314	0.023	0.589
	Immune response	Fatty acid hydrolase domain containing 2	FAXDC2	3.18	5.58	2.395	0.139	0.589
		Guanylate binding protein 1	GBP1	0.19	0.51	0.491	0.493	0.839
		Heat shock protein 1	HSPB1	1.96	1.68	1.909	0.184	0.634
		Peroxisome proliferative activated receptor gamma, coactivator 1 alpha	PPARGC1α	4.06	4.98	1.612	0.220	0.634
		Interleukin 6	IL6	1.07	2.64	3.625	0.073	0.589
		Tumor necrosis factor alpha	TNFα	3.08	5.59	3.413	0.081	0.589
	Digestive enzyme	Aminopeptidase-N	ANPEP	0.82	0.79	0.026	0.873	0.990
		Indoleamine 2,3-dioxygenase	IDO1	3.34	8.33	1.735	0.204	0.634
		Sucrase-isomaltase	SI	9.52	50.86	3.977	0.062	0.589
	Nutrient transporter	Solute carrier family 39 (Zn transporter) member 4	SLC39A4	2.18	2.40	0.255	0.619	0.919
	Stress enzyme	Hydroxysteroid (11-beta) dehydrogenase 1	HSD11B1	2.11	2.92	4.033	0.060	0.589

¹Data are means of 10 newborn piglets for each BW piglet category and per sow experimental diet. Only significant gene expression differences are presented in both treatments (P < 0.10). Gene expression values are presented as ratios of cycle relative threshold value for each gene normalized to that of the reference sample.

2ITM, inorganic trace mineral; Replace, partial replacement of trace mineral source; R&R, reduce and replace mineral source.

³Contrast statistic expresses the variability comparison between the experimental diet and the residual variability within diets.