Table 7.

Relative gene expression differences between light BW piglets of sows fed diets with total ITM and Replace¹

Function	Response	Gene	ITM	Replace	Contrast statistic2	P-value	FDR
Barrier function	Claudin-15	CLDN15	1.36	2.37	6.312	0.022	0.143
	Mucin 2	MUC2	0.87	1.79	10.848	0.004	0.054
	Trefoil factor 3	TFF3	0.93	1.80	13.598	0.002	0.042
	Zonula occludens 1	ZO1	1.06	1.34	4.020	0.060	0.267
Immune response	Fatty acid hydrolase domain containing 2	FAXDC2	1.70	3.17	10.410	0.005	0.054
	Heat shock protein 1	HSPB1	1.27	1.96	18.122	0.0005	0.022
	Peroxisome proliferative activated receptor gamma, coactivator 1 alpha	PPARGC1α	2.87	4.06	3.179	0.091	0.301
	Transforming growth factor beta 1	TGF-β1	1.42	2.03	9.581	0.006	0.057
Antioxidant enzyme	Glutathione peroxidase 2	GPX2	9.08	22.64	3.034	0.099	0.302
	Superoxide dismutase	SOD2	1.17	1.56	7.290	0.015	0.112
Digestive enzyme	Intestinal alkaline phosphatase	ALPI	1.23	1.76	3.595	0.074	0.284
	Aminopeptidase-N	ANPEP	0.61	0.82	3.252	0.088	0.301
Digestive hormone	Cholecystokinin	CCK	0.85	1.35	4.076	0.059	0.267
	Insulin-like growth factor 1 receptor	IGF1R	1.53	2.22	4.756	0.043	0.246
Nutrient transporter	Solute carrier family 39 (Zn transporter) member 4	SLC39A4	1.31	2.18	3.896	0.064	0.267

¹Data are means of 10 newborn piglets per each sow experimental diet. Only significant gene expression differences are presented (P < 0.10). Gene expression values are presented as ratios of cycle relative threshold value for each gene normalized to that of the reference sample.

²Contrast statistic expresses the variability comparison between the experimental diet and the residual variability within diets.