Table 2.
Primer sequences used for real-time PCR
| Items | Primer sequence, 5′ –3′1 |
|---|---|
| IL-1β | F: ACCTGGACCTTGGTTCTC R: GGATTCTTCATCGGCTTC |
| IL-6 | F: GCATTCCCTCCTCTGGTC R: TCTTCAAGCCGTGTAGCC |
| IL-8 | F: TACGCATTCCACACCTTTC R: GGCAGACCTCTTTTCCATT |
| IFN-γ | F: GGCCATTCAAAGGAGCATGG R: GCTCTCTGGCCTTGGAACAT |
| IL-10 | F: TCGGCCCAGTGAAGAGTTTC R: GGAGTTCACGTGCTCCTTGA |
| ZO-1 | F: CTCCAGGCCCTTACCTTTCG R: GGGGTAGGGGTCCTTCCTAT |
| Occludin | F: CAGGTGCACCCTCCAGATTG R: TATGTCGTTGCTGGGTGCAT |
| Claudin-2 | F: GCATCATTTCCTCCCTGTT R: TCTTGGCTTTGGGTGGTT |
| β-Actin | F: GCGTAGCATTTGCTGCATGA R: GCGTGTGTGTAACTAGGGGT |
1F, forward; R, reverse.