Table 3.

Ingredient composition of experimental diets (as-fed basis)¹

	Experimental diet
	Inorganic P			Phytase2
Ingredient, %	0.11	0.19	0.27	150	250	500	1,000	1,500
Basal mix	98.18	98.18	98.18	98.18	98.18	98.18	98.18	98.18
Limestone	0.69	0.77	0.83	0.69	0.69	0.69	0.69	0.69
Monocalcium P	0.16	0.53	0.90	0.16	0.16	0.16	0.16	0.16
Sand3	0.98	0.54	0.10	0.97	0.97	0.97	0.96	0.96
Phytase4	—	—	—	0.0015	0.0026	0.0051	0.0103	0.0154
Total	100	100	100	100	100	100	100	100
Calculated analysis
Crude protein, %	21.7	21.7	21.7	21.7	21.7	21.7	21.7	21.7
Ca, %	0.47	0.60	0.65	0.47	0.47	0.47	0.47	0.47
P, %	0.43	0.51	0.59	0.43	0.43	0.43	0.43	0.43
Phytase, FTU/kg	—	—	—	150	250	500	1,000	1,500
Ca:P ratio	1.10	1.10	1.10	1.10	1.10	1.10	1.10	1.10
Analyzed composition5
Ca, %	0.57	0.63	0.77	0.51	0.53	0.59	0.54	0.54
P, %	0.42	0.47	0.63	0.40	0.43	0.43	0.42	0.41
Phytase, FTU/kg6	—	—	—	137	223	379	919	1,623

¹Diets were fed for 21 d starting at approximately 10 kg.

²GraINzyme (Agrivida Inc., Woburn, MA).

³Sand was used to equalize hand-add batch including the addition of limestone, monocalcium P, and phytase when blended with the basal mix.

⁴Phytase was analyzed and contained 9,738,000 FTU/kg (Agrivida Inc., Woburn, MA).

⁵Complete diet samples were taken during bagging of experimental diets from every fourth bag and pooled into one homogenized sample per dietary treatment. Samples were stored at -20 °C until they were submitted for duplicate analysis of Ca and P (Kansas State University Soils Lab, Manhattan, KS).

⁶Five samples of each diet was submitted to Agrivida Inc. (Woburn, MA) for complete phytase analysis using an adjusted extraction procedure that employed an optimized buffer and extraction process.