FIG. 4.

Cellular turnover of connexin 43 in mice heart. Left panel: Pulse-chase assay reveals different half-lives of Cx43 signals in the triton-soluble (cytoplasmic, interrupted line) and triton-insoluble (membrane-bound, solid line) fractions of Cx43. Right panel: Half-lives of Cx43 in mice ventricular myocytes are about 3.5 h and 5 h for triton-soluble (cytoplasmic) and triton-insoluble (membrane-bound) fractions of Cx43 indicating very rapid turnover. Reproduced with permission from S. Xiao et al., J. Clin. Invest. 130, 9 (2020). Copyright 2020 American Society for Clinical Investigation.²⁸