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. 2021 Jul 1;8:690429. doi: 10.3389/fmolb.2021.690429

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Copyright © 2021 Alexa, Ember, Szabó, Mo’ath, Póti, Reményi and Bánóczi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Internalization and intracellular inhibitory activity of an octaarginine conjugate (3). The conjugate (3) treated cells (20 μM, 5 h) were analyzed by fluorescence microscopy (A), or stimulated by EGF (100 ng/ml). The activity of ERK-pathway was followed by anti-P-ERK antibody using Western-blots (B). The normalized phospho-ERK levels in untreated cells and peptide treated cells are labeled with blue and red, respectively. Error bars show SD calculated based on three independent measurements. * Indicates statistical significance between peptide-treated and control (p < 0.05 with two-sided independent Student’s t-test).