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. 2021 Jul 1;12:651151. doi: 10.3389/fmicb.2021.651151

TABLE 1.

AmpliCoV sequencing of SARS-CoV-2-positive clinical specimens from Germany, direct comparison of rapid library and ligation library.

Sample ID Ct Percentage genome coverage and time of sequencing with depth coverage > 10 after generation of 50,000 reads per sample
Percentage genome coverage (length) Time of sequencing (hours) Percentage genome coverage (length) Time of sequencing (hours)


Rapid library* Ligation library*
AC_01 18.1 98.4 02:42:41 97.3 00:53:15
AC_02 18.4 97.3 01:45:58 96.4 00:40:15
AC_03 24.7 94.4 02:42:35 91.9 00:31:16
AC_04 23.3 96.0 02:41:20 93.6 00:44:32
AC_05 17.7 96.9 01:44:46 95.9 00:40:09
AC_06 21.9 96.0 01:57:23 94.1 00:42:05
AC_07 23.2 95.0 02:33:40 95.0 00:35:09
AC_08 22.1 92.0 01:39:19 92.6 00:40:29

*Multiplexing of 11 samples on one flow cell.