TABLE 1.
AmpliCoV sequencing of SARS-CoV-2-positive clinical specimens from Germany, direct comparison of rapid library and ligation library.
| Sample ID | Ct | Percentage genome coverage and time of sequencing with depth coverage > 10 after generation of 50,000 reads per sample |
||||||
| Percentage genome coverage (length) | Time of sequencing (hours) | Percentage genome coverage (length) | Time of sequencing (hours) | |||||
| Rapid library* | Ligation library* | |||||||
| AC_01 | 18.1 | 98.4 | 02:42:41 | 97.3 | 00:53:15 | |||
| AC_02 | 18.4 | 97.3 | 01:45:58 | 96.4 | 00:40:15 | |||
| AC_03 | 24.7 | 94.4 | 02:42:35 | 91.9 | 00:31:16 | |||
| AC_04 | 23.3 | 96.0 | 02:41:20 | 93.6 | 00:44:32 | |||
| AC_05 | 17.7 | 96.9 | 01:44:46 | 95.9 | 00:40:09 | |||
| AC_06 | 21.9 | 96.0 | 01:57:23 | 94.1 | 00:42:05 | |||
| AC_07 | 23.2 | 95.0 | 02:33:40 | 95.0 | 00:35:09 | |||
| AC_08 | 22.1 | 92.0 | 01:39:19 | 92.6 | 00:40:29 | |||
*Multiplexing of 11 samples on one flow cell.