Figure 3.

Semi-quantitative RT-PCR analysis of IRT1. (a) Long IRT1 splicing form was detected with three independent primers combinations in iron-deficient seedling roots but not leaves after 35 cycles. (b) After 50 cycles long splicing form was also detected in aboveground organs. We failed to distinguish genomic contamination in cDNA samples. Bands obtained from genomic DNA under the same PCR conditions are presented for fragment size comparison. (c) Short IRT1 splicing form was detected in seedling leaves and roots regardless of iron status and in all aboveground organs of mature plants (d). (e and f) represent reference transcripts after 35 cycles