Figure 4.

Western blotting IRT1 detection. (a) IRT1 protein abundance in aboveground parts of plants grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle membrane fraction, PM = plasma membrane fraction