Table 1.

The sequences of DNA oligomers used in this study

Oligomer	Sequence
For pAtIRT1::GUS construct:
pAtIRT1-BglII-F	5′-CACAGATCTTCAAATCATACACACTTCATCCTG-3′
pAtIRT1-XmaI-R	5′-TTTCCCGGGTTTTTTTTTTTTTCTTTTTTCTTTTGGATTG-3′
pAtIRT2-BglII-F	5′-GACAGATCTAACTTCTAAACTCTTCCTCATCTC-3′
pAtIRT2-XmaI-R	5′-TATCCCGGGGTAGTATTGAGATTGTTTTATAATATATGATG-3′
For pAtIRT1::gAtIRT1::Dendra2 constructs:
gIRT1.2-PstI-R	5′-ATTAGCTGCAGAGCCCATTTGGCGATAATCGACATTC-3′
gIRT1.1-PstI-R	5′-GTAATCTGCAGTTTAAAATACAATGATATTCGGTATGTATA-3′
Dendra2-PstI-F	5′-GATACTGCAGGGAGCTGGTATGAACACTCCTGGAATCAATCTC-3′
Dendra2-BamHI-R	5′-CAAGGATCCTCACCAAACCTGTGATGGGAGAG-3′
pAtIRT1-AscI-F	5′-CGGGAGGCGCGCCAAATCATACACACTTCATCCTGACAA-3′
For RT-PCR:
IRT1.1–279/2-313-F	5′-TCGTAATCCTCATTGCAAGCATGA-3′
IRT1.1–415/2-449-F	5′-TATGCACGTTTTACCTGATTCTTTC-3′
IRT1.1–551/2-585-F	5′-ACGAGCCTATACACCAGCAAGA-3′
IRT1.2/1368-R	5′-TGGGGCCTATCACATTTACATATCTACC-3′
IRT1.2/1149-R	5′-CGAGAAGAGCCGCGATTAAACA-3′
IRT1.1/723-R	5′-CGGTATGTATATATGTGCGTACGAACC-3′
IRT1.2/753-R	5′-CTATGATCCCAAGTTCCAAGACC-3′
IRT1.1/746-R	5′-GTCATTTAAAATACAATGATATTCGGTATGTA-3′
AT4G05320.1–908-F	5′-GGAAAACAATTGGAGGATGGTC-3′
AT4G05320.1–1238-R	5′-ACGAGATTTAGAAACCACCACGA-3′
AT2G28390-1905-F	5′-AAAGGATTGGGACCCCACAA-3′
AT2G28390-2250-R	5′-TCTCTCAAGGGTTTCTGGGTACA-3′