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. 2021 Jul 15;89(8):e00111-21. doi: 10.1128/IAI.00111-21

FIG 9.

FIG 9

Human corneal cell susceptibility and cytokine response to S. marcescens. (A) Cell viability was evaluated following 2-h challenge by S. marcescens and mutants at MOIs of 2, 20, 50, and 200. At an MOI of 2, the ΔrcsB and ΔgumB ΔrcsB mutants were more cytotoxic than the WT. At all MOIs, the ΔgumB, ΔshlB, and ΔrcsB ΔshlB mutants were less cytotoxic than the WT. n, 3. (B) IL-1β analysis of HCLE cells exposed to normalized bacterial supernatants for 5 h and measured using BioLegend flow-based ELISA. n, 12. Means and SD are shown. Asterisks indicate significant differences by ANOVA with Tukey's posttest. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.