FIG 1.

C. trachomatis aberrant body formation results in cell division arrest and pausing of the developmental cycle. (a) Diagram of the Chlamydia developmental cycle. (b) Volume measurements of C. trachomatis present within inclusions at 24 hpi under untreated and aberrance-inducing conditions. Data were collected by fluorescence microscopy over three independent experiments. Each data point represents the collective total of contiguous, MOMP-labeled material (∼100 inclusion measurements taken per group). (c to e) Electron micrographs of HeLa cells infected with C. trachomatis at 24 hpi that were either untreated (c) or treated with AMP (d) or the iron chelator DPP (e). Black arrowheads indicate RBs and ABs, and red arrowheads indicate the much smaller, electron-dense EBs. Bars, ∼2 μm. (f to h) Inclusion-forming unit (IFU) counts were conducted at 44 h postinfection/posttreatment (f) and after recovery in the absence of treatment for an additional 24 h (g) and 48 h (h). The dotted lines represent the assay’s limit of detection. C1, T3SS inhibitor compound C1; MP265, MreB polymerization inhibitor. Each data point plotted represents data from an independent experiment, and error bars represent the standard deviations from the means.