FIG 4.

The presence of PG and differences in cell/inclusion size are dependent on the timing of persistence induction. (a) Chlamydia-infected HeLa cells were grown in the presence of the PG-labeling reagent EDA-DA for 24 h. (b to d) Persistence was induced with the T3SS inhibitor compound C1 at 2 hpi (b), 8 hpi (c), and 22 hpi (d). (e to g) Enhanced/magnified regions of interest from panel d. Arrowheads indicate discernible breaks in the RB PG rings. Images are representative of 20 to 30 viewing planes over three separate and independent experiments. Bars, ∼1 μm.