FIG 6.

Chlamydia ABs differ in their presentations of the late-stage inclusion membrane protein CT813. HeLa cells were infected at an MOI of 1. At 2 hpi, cells were either left untreated or treated with various aberrance inducers, and cells under all conditions were fixed at 24 hpi. Immunolabeling was conducted for the inclusion membrane protein CT813 as well as MOMP. (a) Ampicillin (AMP) and DPP treatments. See Fig. S4 in the supplemental material for the rest of the conditions. Bars, ∼2 μm. Images are representative of between 10 and 20 inclusions observed under each condition over the course of three separate experiments. To assess actin cage formation, HeLa cells were infected at an MOI of 1, fixed at 46 hpi, and labeled for MOMP and phalloidin. (b) AMP and DPP treatments. The rightmost panels are magnifications of the boxed areas. The remaining aberrance-inducing conditions are presented in Fig. S5 in the supplemental material. Images are representative of 20 fields of view under each condition examined over three separate experiments. Bars, ∼5 μm.