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. 2021 Jul 12;8(4):ENEURO.0559-20.2021. doi: 10.1523/ENEURO.0559-20.2021

Figure 3.

Figure 3.

Postmortem analysis of pre-L-DOPA miRNA Fyn treatment. A, TH-positive cell counting at the SNpc from immunohistochemical staining of coronal slices. One-way ANOVA (F(3,27) = 1.344, p = 0.2809). B, Analysis of dopamine depletion by Western blot quantification of TH/β-actin. Kruskal–Wallis test H(3) = 11.99; p = 0.0074 followed by Dunn’s test (p = 0.0332, no LV vs NR). C, FosB-ΔFosB levels relative to β-actin. One-way ANOVA (F(3,26) = 3.583, p = 0.0272) and post hoc Tukey’s test (p = 0.0236, R vs NR). D, Detection of neuronal Fyn activity by Western blot quantification of the phosphorylation status of NR2B in striatal homogenates. Values indicate pNR2B/NR2B/β-actin ratio. One-way ANOVA (F(3,20) = 8.035, p = 0.0010) and post hoc Tukey’s test (p = 0.0114 and p = 0.0006, for no LV vs NR and R, respectively). In all figures, data are mean ± SEM. In B–D, the black bar indicates mean ± SEM of the contralateral non-lesioned striatal samples, as a reference value and was not included in the statistical analysis. Figure Contributions: Juan E. Ferrario and Sara Sanz-Blasco dissected brain structures. Melina P. Bordone and Tomas Eidelman performed mesencephalic slices and immunohistochemistry of TH. Tomas Eidelman quantified TH-positive cells and Melina P. Bordone made Western blottings.