Table 1.
Distribution of MICs of tested antibiotics among phenotypically resistant LAB strains (n = 34).
| Number | Strain | MIC (mg/L)a | |||||||
|---|---|---|---|---|---|---|---|---|---|
| GMb | K | TE | CH | A | E | CL | S | ||
| Microbiological cut-off values (mg/L) proposed by EFSA for obligate heterofermentative Lactobacillus | |||||||||
| 16 | 64(32)c | 8 | 4 | 2 | 1 | 4(1) | 64 | ||
| 1 | L. buchneri KKP 2047p | 4 | 128 | 16d | 4 | 1 | 0,25 | 0,5 | 64 |
| 2 | L. diolivorans KKP 2036p | 8 | 128 | 16 | 2 | 2 | 0,25 | 0,125 | 128 |
| 3 | L. fermentum KKP 2020 | 2 | 32 | 16 | 4 | 0,5 | 0,25 | 0,25 | 32 |
| 4 | L. fermentum KKP 830 | 8 | 64 | 16 | 2 | 1 | 0,25 | 0,25 | 64 |
| 5 | L. fermentum Sieger | 16 | 128 | 4 | 4 | 0,125 | 0,125 | 0,03 | 32 |
| 6 | L. brevis Pap3/4 | 2 | 64 | 16 | 4 | 0,25 | 0,125 | 0,25 | 64 |
| 7 | L. brevis Pat1 | 0,5 | 16 | 16 | 4 | 2 | 0,5 | ≤0,015 | 8 |
| 8 | L. brevis Solaris | 1 | 16 | 16 | 4 | 1 | 0,25 | 0,5 | 16 |
| 9 | L. farraginis E/J | 0,5 | 8 | 16 | 4 | 0,125 | 0,03 | 0,03 | 8 |
| Microbiological cut-off values (mg/L) proposed by EFSA – facultative heterofermentative Lactobacillus | |||||||||
| 16 | 64 | 8 | 4 | 4 | 1 | 4(1) | 64 | ||
| 10 | L. agilis KKP 1834 | 32 | ≥256 | 0,25 | 4 | 1 | ≥8 | 1 | ≥256 |
| 11 | L. salivarius KKP 1828 | 16 | 128 | 1 | 4 | 0,25 | 0,125 | 0,125 | 128 |
| 12 | L. salivarius KKP 1835 | 8 | 128 | 16 | 4 | 2 | 0,125 | 0,03 | 128 |
| Microbiological cut-off values (mg/L) proposed by EFSA for Lactobacillus rhamnosus | |||||||||
| 16 | 64 | 8 | 4 | 4 | 1 | 4(1) | 32 | ||
| 13 | L. rhamnosus KKP 849 | 4 | 128 | 1 | 8 | 1 | 0,5 | 0,5 | 32 |
| 14 | L. rhamnosus B/J | 32 | 128 | 1 | 4 | 0,5 | 0,125 | 0,5 | 32 |
| Microbiological cut-off values (mg/L) proposed by EFSA for Lactobacillus plantarum/pentosus | |||||||||
| 16 | 64 | 32 | 8 | 2 | 1 | 4(2) | n.r. | ||
| 15 | L. plantarum KKP 804 | 4 | 64 | 32 | 4 | ≥8 | 0,25 | 4 | n.r. |
| 16 | KKP 815 | 8 | 128 | 16 | 8 | 1 | 0,25 | 2 | n.r. |
| 17 | KKP 835 | 8 | ≥256 | 16 | 8 | 2 | 0,25 | 1 | n.r. |
| 18 | KKP 870 | 16 | ≥256 | 32 | 8 | 2 | 0,25 | 4 | n.r. |
| 19 | KKP 872 | 16 | ≥256 | 16 | 8 | 2 | 0,25 | 4 | n.r. |
| 20 | KKP 2021p | 4 | 128 | 16 | 8 | 1 | 0.25 | 4 | n.r. |
| 21 | KKP 1821 | 4 | 128 | 16 | 4 | 1 | 0,25 | 0,5 | n.r. |
| 22 | KKP 1822 | 8 | 128 | 16 | 8 | 1 | 0,25 | 0,5 | n.r. |
| 23 | ATTC 8287 | 8 | 128 | 16 | 8 | 2 | 0,5 | 2 | n.r. |
| Microbiological cut-off values (mg/L) proposed by EFSA for obligate homofermentative Lactobacillus | |||||||||
| 16 | 16 | 4 | 4 | 2(1) | 1 | 4(1) | 16 | ||
| 24 | L. delbrueckii PCM 490 | 4 | 32 | 2 | 2 | 0,06 | 0,06 | 0,06 | 8 |
| Microbiological cut-off values (mg/L) proposed by EFSA for Lactobacillus acidophilus group | |||||||||
| 16 | 64 | 4 | 4 | 1 | 1 | 4(1) | 16 | ||
| 25 | L. acidophilus PCM 2499 | 4 | 16 | 32 | 2 | 0,25 | 1 | 0,125 | 32 |
| 26 | L. johnsonii KKP 878 | 4 | 64 | 16 | 8 | 0,125 | 0,25 | 0,5 | 32 |
| Microbiological cut-off values (mg/L) proposed by EFSA for Pediococcus spp. | |||||||||
| 16 | 64 | 8 | 4 | 4 | 1 | 1 | 64 | ||
| 27 | P. pentosaceus KapA | 4 | 128 | 16 | 4 | 2 | 0,25 | 0,03 | 128 |
| 28 | P. pentosaceus Pom7 | 4 | 64 | 16 | 2 | 1 | 0,25 | 0,03 | 64 |
| 29 | P. pentosaceus AG | 16 | 128 | 16 | 4 | 2 | 0,5 | 0,03 | 128 |
| 30 | P. pentosaceus MA | 16 | ≥256 | 16 | 4 | 2 | 0,25 | 0,03 | 64 |
| 31 | P. pentosaceus WN1 | 8 | 64 | 16 | 4 | 1 | 0,5 | 0,03 | 128 |
| 32 | P. acidilactici KKP 1839 | 4 | 128 | 16 | 4 | 2 | 0,25 | 0,03 | 128 |
| Microbiological cut-off values (mg/L) proposed by EFSA for Enterococcus spp. | |||||||||
| 32 | 1024 | 4 | 16 | 2 | 4 | 4 | 128 | ||
| 33 | E. durans KKP 1586 | 16 | 64 | 0,5 | 8 | 0,25 | ≥8 | ≥8 | 128 |
| 34 | E. faecium TR2 | 32 | 128 | 32 | 2 | 0,125 | ≥8 | 4 | 128 |
a
MICs higher than EFSA cut-off values in bold;
b
GM, gentamicin; K, kanamycin; TE, tetracycline; CH, chloramphenicol; A, ampicillin; E, erythromycin; CL, clindamycin; S, streptomycin;
c
the previous EFSA proposed cut-off values (2012) are given in brackets;
d
L. buchneri the cut-off for tetracycline is 128; KKP - strains from the Culture Collection of Industrial Microorganisms; PCM - strains from The Polish Collection of Microorganisms; n.r., not required.