FIG 2.

Pan-HIF signaling is preferentially activated at the site of infection following L. major inoculation in vivo. C57BL/6 mice were infected with 2 × 10⁶ L. major parasites intradermally in the ear. At 2 to 4 weeks p.i., infected and contralateral ears and dLNs were digested and mRNA was extracted from total ear cells. The expression of Hif1α (A), Hif2α (B), Vegfa (C), Nos2 (D), Pgk1 (E), Ldha (F), Arg1 (G), Epo (H), Oct4 (I), and Angpt2 (J) was analyzed by quantitative real-time PCR. Relative mRNA expression normalized to the housekeeping gene RpsII is presented as the mean + SEM. Results shown here are from 1 representative experiment of 3 individual experiments that included 3 to 9 mice per group. *, P ≤ 0.05; **, P < 0.01; ***, P < 0.001, t test comparing ears to dLNs.