FIG 5.

Myeloid pan-HIF signaling promotes lymphangiogenesis during L. major infection. LysM^Cre ARNT^f/f or LysM^Cre ARNT^f/+ mice were infected with 2 × 10⁶ L. major metacyclic parasites intradermally in the ear. Ears were analyzed at 4 weeks p.i. (A and B) H&E-stained infected LysM^Cre ARNT^f/+ and LysM^Cre ARNT^f/f ears. (C and D) Infected LysM^Cre ARNT^f/+ and LysM^Cre ARNT^f/f ears were stained for LYVE-1 to delineate lymphatic ECs and lymphatic vessels by immunofluorescence microscopy. (E and F) Magnified view of LYVE-1⁺ lymphatic vessels from images C and D. (G) LYVE-1 expression was quantified by immunofluorescence microscopy. (H) Lymphatic vessel density was quantified by microscopy. *, P ≤ 0.05, t test comparing infected LysM^Cre ARNT^f/f mice to infected LysM^Cre ARNT^f/+ controls. Data are presented as mean + SEM. The data presented were from 2 pooled experiments with a combined total of 8 mice per group. Scale bars: 300 μm (C and D) and 100 μm (E and F).