Figure 1.

LDHA expression is increased in endometriotic lesions. (A) Representative immunohistochemistry photomicrographs of LDHA in normal endometrium (n=35), as well as in eutopic (n=39) and ectopic tissues (n=37). (B) Summarized H-scores of LDHA protein expression in endometrial epithelial cells and ESCs in the three sample types are shown. H-scores are expressed as medians and interquartile range (25th-75th percentile). (C) LDHA expression in primary cultured ESCs was determined by reverse transcription-quantitative PCR (n=10). (D) Western blot analysis of LDHA expression in primary cultured ESCs (n=4). Values are expressed as mean ± SD of three independent experiments. One-way ANOVA and Bonferroni post hoc test were used to analyze multiple comparisons. For H-Score data, Kruskal-Wallis and Bonferroni post hoc tests were applied. *P<0.05, **P<0.01 and ***P<0.001. LDHA, lactate dehydrogenase A; ESCs, endometrial stromal cells; NESC, normal endometrial stromal cells; EuESC, eutopic endometrial stromal cells; EuESC, eutopic endometrial stromal cells.