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. 2021 Jul 6;24(3):637. doi: 10.3892/mmr.2021.12276

Figure 4.

Figure 4.

Effect of LDHA on apoptosis of immortalized human endometrial stromal cells (THESC cells). (A) Representative graphs of apoptosis detected by flow cytometry. (B) An increased number of THESC cells underwent apoptosis when LDHA was knocked down (sh-LDHA) compared with the control (sh-Con). (C) Western blot analysis of apoptosis-related proteins. (D) Histogram of BID, BAX, BIK, P-BAD/BAD, C-caspase 3, total caspase 3 and LDHA protein expression in THESC cells. Values are expressed as mean ± SD of three independent experiments. Student's t-test was used to compare two groups. *P<0.05 and ***P<0.001. LDHA, lactate dehydrogenase A; sh-, short hairpin; Con-, control; C-, cleaved; P-, phosphorylated.