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. 2021 Jul 1;9:688467. doi: 10.3389/fbioe.2021.688467

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Copyright © 2021 Certelli, Valente, Uccelli, Grosso, Di Maggio, D’Amico, Briquez, Hubbell, Wolff, Gürke, Mujagic, Gianni-Barrera and Banfi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Long-term maturation and size of angiogenic vessels in diabetic skin. (a–c) Vascular morphology was analyzed 4 weeks after implantation of V, VP or control (ctrl) empty fibrin gels, by immunofluorescence staining for podocalyxin (endothelial cells, red), NG2 (pericytes, green), α-SMA (smooth muscle cells, cyan), and DAPI (nuclei, blue). The lower panels show higher-magnification views of the areas marked by the white squares in the top panels. Size bars = 50 μm in all panels; dWAT, dermal adipose white tissue; PC, panniculus carnosus; ICT, interstitial connective tissue. (d,e) Vessel diameters (in μm) were quantified and results are shown as mean values of each sample ± SEM (d) and as the distribution of vessel diameters over 1-μm intervals (e); n = 6–9 samples/group.