Figure 3.

ANXA1sp treatment limits oxidative damage and improves markers of mitochondrial integrity and function. Mice were treated with either Vehicle or ANXA1sp 1 h prior to ischemia, subjected to 33 min of unilateral ischemia and contralateral nephrectomy, and then re-injected with Vehicle or ANXA1sp 1 h after reperfusion. Kidney tissues were harvested at 24 h after reperfusion. (A) Representative immunofluorescence histologic staining for 8-OHdG and citrate synthase. (B) Protein levels of superoxide dismutase 2 (SOD2) were determined by Western blot with densitometry shown in the graph below. (C) Protein levels of 8-Oxoguanine DNA Glycosylase (Ogg1) were determined by Western blot with densitometry shown in the graph below. (D) Mitochondrial protein levels of mitochondrial transcription factor A (Tfam) were determined by Western blot with densitometry shown in the graph below. Graphs display mean +/-SEM of densitometry of protein normalized to the loading control. Statistical significance determined by two-way ANOVA with Sidak post-test (n = 3 samples for Sham groups, n = 6 samples for I/R groups; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001).