Figure 3.

CIN attenuates oxidative stress damage in M5-stimulated NHEKs. NHEKs were treated with CIN (5 or 10 µM) for 24 h, and then treated with 10 ng/ml M5 for 24 h. (A) Flow cytometry was used to measure the production of intracellular ROS in NHEKs. (B) ELISA was used to detect the levels of MDA and GSH in the supernatants of NHEKs. n=3. *P<0.05, **P<0.01 vs. control group; ^##P<0.01 vs. 10 ng/ml M5 group. CIN, cinnamaldehyde; NHEKs, normal human epidermal keratinocytes; ROS, reactive oxygen species; MDA, malondialdehyde; GSH, glutathione.