Figure 6.

CIN inhibits the proliferation and inflammation of M5-treated NHEKs via downregulation of NF-κB and JNK signaling pathways. NHEKs were treated with CIN (5 or 10 µM) for 24 h, and then treated with 10 ng/ml M5 for 24 h. (A-D) Western blotting was performed to measure the expression levels of p-IκBα, p-p65 and p-JNK in NHEKs. The relative expression levels of p-IκBα, p-p65 and p-JNK in NHEKs were normalized to IκBα, p65 and JNK. n=3. **P<0.01 vs. control group; ^##P<0.01 vs. 10 ng/ml M5 group. CIN, cinnamaldehyde; NHEKs, normal human epidermal keratinocytes; p-, phosphorylated; IκBα, inhibitor of NF-κB.