Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 1;9:668715. doi: 10.3389/fcell.2021.668715

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Zhang, Hu, Ding, Yuan, Jia, Wu and Cheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CircSNHG5 serves as a sponge for the miR-495-3p to inhibit its expression. (A) The expression of predicted miRNAs in chondrocytes after transfection with siRNA (*P < 0.05, ***P < 0.001). (B–D) qRT-PCR analysis confirmed the upregulated miRNAs in different CEP tissues of 21 patients and 21 control samples (***P < 0.001). (E) RNA FISH detection of the subcellular localizations of circSNHG5 and miR-495-3p. Both molecules were co-localized and both were cytoplasmic. miR-495-3p probes were labeled with Cy3, whereas circSNHG5 probes were tagged with Alexa fluor 488. Scale bar, 20 μm. (F) The binding region of miR-495-3p in circSNHG5 3′UTR is shown. (G) Luciferase reporter analysis of either wild-type or mutant circSNHG5 3′-UTR activity. miR-495-3p was co-transfected with the wild-type or mutant vector. The presented values are the mean ± SEM of three different preparations (**P < 0.01).