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. 2021 Jul 1;9:668715. doi: 10.3389/fcell.2021.668715

FIGURE 4.

FIGURE 4

Function of miR-495-3p in chondrocytes and miR-495-3p inhibitor reversed the effects of siRNA. (A) The expression of miR-495-3p was assessed by qRT-PCR and transfected with miR-495-3p mimics and miR-495-3p inhibitor (∗∗P < 0.01, ∗∗∗P < 0.001). (B) The effect of miR-495-3p overexpression on cell proliferation in vitro was determined by CCK8 assay (∗P < 0.05). (C) Western blot analysis of MMP13, Collagen II, and Aggrecan transfected with miR-495-3p mimics. The optical density analysis was performed from the results of three independent experiments of Western blot samples (∗P < 0.05, ∗∗P < 0.01). (D) Relative mRNA expression test by qRT-PCR (∗∗P < 0.01, ∗∗∗P < 0.001). (E) Chondrocytes were co-transfected with a miR-495-3p inhibitor and siRNA; cell proliferation was monitored by CCK-8 assay (∗P < 0.05, ∗∗P < 0.01). (F) Western blot analysis of MMP13, Collagen II, and Aggrecan co-transfected with a miR-495-3p inhibitor and siRNA. The optical density analysis was performed from the results of three independent experiments of Western blot samples. (G) Relative mRNA expression test by qRT-PCR (∗P < 0.05).